An experimental and analytical investigation using Fourier Transform Technique (FTT) and Laplace Transform Technique (LTT) were applied to predict contaminants concentration along transient groundwater flow in homogeneous, semi-infinite aquifer. The solutions were obtained for sinusoidal and exponential form of velocity which represent the seasonal pattern in a year. MatLab software was used to determine values of solute concentration for different time intervals and different distances with the same initial and boundary conditions. The analytical concentration values were compared with the experimental obtained concentration. It was observed that the experimental pollutant concentration trends are consistent with the analytical simulations of the Laplace transform technique (LTT) and the Fourier transform technique (FTT). A new empirical equation was proposed to calculate a new concentration for all samples and all experimental parameters. The obtained new concentration results were in good agreement with both the Fourier Transform Technique (FTT) and the Laplace Transform Technique (LTT).