Intestinal parasite infection is one of the most important health problems facing the world. It is widespread in tropical and subtropical regions due to their favorable climatic conditions for the continuity and development of the stages that parasites go through during their life cycle. This study included 300 stool samples from patients visiting Yafran Central Hospital and Zintan Hospital during the period from January 2022 to October 2023 to identify intestinal parasites and to study the prevalence of some intestinal parasites in the cities of Zintan and Yafran. The results indicated that 36.67% of all cases in the cities of Yafran and Zintan had infections from intestinal parasites Entamoeba histolytica, Entamoeba coli, Giardia lamblia, and Enterobius vermicularis. The infection rate in males was 20.00%, while the infection rate in females was 16.67%. The infection rate in males in Zintan city was higher than in Yafran city (11.67% and 8.33%), and also in females (10.00% and 6.67%), respectively. It was noted that the infection rate with intestinal parasites in Zintan city was higher at 21.67% compared to Yafran city at 15.00%. The results showed that the age group 1-10 years was the age group most affected by intestinal parasites, with a percentage of 12.33%, followed by the age group less than one year old, with a percentage of 6.33%. The age group 61 years and older had the lowest percentage of intestinal parasites, with a percentage of 0.33%. According to the infection with intestinal parasites, the highest infection was with Entamoeba coli at 16.7%, followed by Entamoeba histolytica at 13.00%, and the least infection was with Giardia lamblia and Enterobias vermicularis at 1%. There were mixed cases infected with E. histolytica and E. coli at 5.00%. Keywords: Intestinal parasites, E. histolytica, G. lamblia