The thyroid hormones regulate synthesis, mobilization, and breakdown of lipids. Lipid profiles are often negatively altered by thyroid dysfunction, which is becoming increasingly prevalent. Objectives: The current study aimed to evaluate the association between serum levels of thyroid-stimulating hormone and thyroid hormones and the lipid profile in patients with thyroid disorders. Materials and Methods: The study included 100 participants, aged between 18 and 65 years. Participants were recruited from endocrinology clinics at hospitals in Al-zawia, Surman, Sabratha, and the neighboring regions. Participants were divided into two groups: Group (1): 50 healthy individuals as control. Group (2): 50 patients with thyroid dysfunction. A 5 ml blood sample was collected from each participant after 12 hours of fasting. TSH, T4 & T3 hormones, total cholesterol, triglycerides, HDL, LDL, and VLDL were measured. The associations between TSH levels and thyroid hormone and lipid profile levels were assessed using the Chi-square test, t-test, and Spearman correlation using SPSS version 26. Results: The distribution of thyroid disorder patients according gender were 86% females and 14% males, according age groups were 04%, 10%, 36%, and 50% in age groups ≤30, 31-40, 41-50, and >50 years, respectively, according body weight groups were 06%, 56%, and 38% in body weight groups ≤60, 61-90, and >90 kg, respectively. 46% of the thyroid disorder patients had chronic diseases, including 22% hypertension, 20% diabetes mellitus, 6% heart diseases, 6% hemodialysis, and 4% cancer. The mean of TSH levels was significantly (P<0.05) increased in patients compared with the control group, was significantly (P<0.01) increased in the hypothyroidism group compared with the euthyroid group in thyroid disorder patients, but significantly (P<0.01) decreased in females compared with male thyroid disorder patients. The mean of T3 levels was significantly (P<0.01) decreased in patients compared with the control group. The mean of T4 levels was significantly (P<0.01) decreased in the hypothyroidism group compared with the euthyroid group. The levels of lipid profiles were showed variations and correlations with TSH, T3, T4. Conclusion: It can be concluded that the thyroid dysfunction patients were 86% female, 50% in the age group >50 years, 56% and 38% in body weight groups 61-90 and >90 kg, 62% had secondary education. The mean duration of thyroid disorder and medication was 5.8 ± 1.2 and 5.0 ± 1.1 years. The levels of thyroid hormones and lipid profiles were showed variations and correlations with each other......................... Keywords: ...............Thyroid disorders, TSH, T3, T4, Lipid Profile, Correlation, Surman region, Western Libya