This study was conducted to determine the prevalence of Entamoeba histolytica parasite in the western region of Libya. and to determine the city where the parasite is most prevalent in addition to studying the relationship between infection and gender infection and age and which age groups are most affected 600 stool samples were examined from people visiting al-Zawia Teaching Hospital and the Surman central laboratory, in addition to the preventions laboratories in the city of Sabratha, during the period from January to December 2024. the samples were distributed equally, 200 samples for each city randomly and the sample included all age groups without exception. The results of the study showed the prevalence rate of with the parasite Entamoeba histolytica was (29.2%). The city of Sabratha recorded an infection rate of (11.5%), Followed by the city of Zawia (9.7%), then Surman with a rate (9.0%).There is no significant differences in infection rates between regions (p=0.611, x² = 0.984). The percentage of infected males was (15.8%) and the percentage of infected females was (13.3%), there is no statistically significant relationship between gender and infection (p=0.724, x² = 0.124) The results showed that the two age groups, 40 years and over and 10 years and less, are the most infected groups, with the parasite with percentages of (11.2%) and (9.5%), respectively. The results indicate that there is no significant relationship between age and the likelihood of infection with the parasite (p=0. 160, x² = 3.662).................. Keywords: ..............parasite, Entamoeba histolytica, prevalence of infection, western region of Libya.