المجلة الدولية للعلوم والتقنية

Hemodialysis is a life-saving procedure in patients with chronic renal failure (CRF). However, in this procedure, infections, which often cause patient deaths, may develop due to catheter application. There were no data regarding catheter-related bloodstream infections (CRBSIs) in hemodialysis in Libya. In this study, we aim to study: To identify complications in patients with chronic renal failure associated with central venous catheter related infections in patients, undergoing hemodialysis, and to identify micro-organisms species, as well as their susceptibility profiles to the most commonly used antimicrobial agent. We assessed common study testswas performed at the Microbiology Laboratory, from September to October 2022. 65 patients undergoing hemodialysis, at Janzour kidney service center, Tripoli, Libya.Were included in this research. Blood samples were collected from central venous catheter (CVC), and fistula-associated arteriovenous (AVF). The samples were cultured according to the standard microbiological procedures. Isolates were identified by conventional identification methods. Data was submitted to all patients to collect information such as age, gender, and health condition. We retrospectively examined65 patients hemodialysis, 33(51%) were female, and 32 (49%) were male. The mean age of the patients was 37.82 years ± 17.428 SD (range: 5-78). Hemodialysis access route was AVF in 55 patients (84.6%), and CVC in 10 patients (15.4%). Patients with use catheter days had higher cases of central venous catheter (CVC) 5(62.5%) compared to patients with was arteriovenous fistula (AVF) 3 (37.5%).Of the 65 blood cultures, 8(12.3%) tested positive. Among all microorganisms isolated, Candida albicans 2(25%) in the central venous catheter, and 1(12.5%) in the arteriovenous fistula. The second isolate the gram-positivebacteria were Staphylococcus aureus 3(37.5 %) in the central venous catheter, and gram-negative isolates organisms were Escherichia coli 2 (25%),) in the arteriovenous fistula. Identified bacteria isolates were tested for susceptibility to twelve antibiotics, high resistance in gram-negative isolates collection. E. coli was 100% resistant to CIP, AMP. Staphylococcus, aureus isolates showed extreme resistance against CFM, CL, IMI, C-5, MRP, F-300 and TOP 100%, 75%, respectively. In conclusion, the fistula is the best available option for hemodialysis patients, with a much lower infection compared to patients with wasCentral venous catheter. A longitudinal study with comparison of multiple units representing different healthcare sectors would improve our knowledge on risk factors and practices associated withblood stream infection impact of infecting (BSIs) among hemodialysis patients in Libya. Keywords: CVC, AVF, Bloodstream infections, Antimicrobial.