المجلة الدولية للعلوم والتقنية

Pathogenic bacteria pose a serious risk in schools, where students come into contact with a variety of microbes on a daily basis. Classrooms are thought to be ideal environments for young children to harbor germs. The current study sought to determine the extent of the spread of bacteria in different places in primary schools, as well as to determine whether the schools are sterile and clean for children or whether they are the real cause of their illness. Samples were taken from the primary schools. The schools are located in Wadi Jaref ,Sirte. 30 samples were collected from different classrooms and from places the student comes into contact with, such as door handles, desk, and children’s hands. The organisms isolated in this study, based on colonial morphological, and biochemical characteristics the results showed the presence of bacteria Staphylococci epidermidis(35%), Staphylococcus aureus (22%), Esherichia coli (13%), klebsiella pp, micrococcus and Streptococcus (9%), bacillus(4%). The study showed that the largest number of bacterial colonies (Colony Forming Units) (CFU) found on the hands (75 CFU), Desks(26 CFU) and Door handles (22 CFU). This study emphasized the wide range of possible diseases and demonstrated the presence of harmful bacteria in primary schools. This is related to the increasing number of students in primary schools which lack proper hygiene conditions and due to the failure to carry out routine cleaning and sterilization of schools and the lack of facilities................ Keywords:.. Primary schools, Bacteria, Contamination, Microorganisms, Children, Colony, Wadi Jaref, Sirte.